Journal: JCI Insight

Article Title: Role of cGAS/STING pathway in aging and sexual dimorphism in diabetic kidney disease

doi: 10.1172/jci.insight.174126

Figure Lengend Snippet: ( A ) Representative images of immunohistochemical staining of kidneys for STING in healthy and type 2 diabetic humans (left), nondiabetic (Wistar) rats, and rats with type 2 diabetic neuropathy (T2DN) (right). G, glomerulus. Scale bars: 150 μm. ( B ) Examination of CD68 and STING by immunofluorescence microscopy in diabetic human kidney. Scale bars: 50 μm (left) and 5 μm (right). ( C ) Immunohistochemical detection of CD68 in kidneys of old male Wistar and T2DN rats. Scale bar: 150 μm. ( D ) Representative 3D image from a human kidney stained for inactive and active STING molecules. Scale bar: 2 μm. ( E ) Immunohistochemical staining for mtTFA and TREX1 in renal cortical sections from healthy and diabetic human kidneys. Scale bars: 150 μm. ( F ) Western blot analysis of mtTFA and TREX1 in the kidneys of Wistar and T2DN old male rats. n = 6 rats in each group. β-Actin was used as a loading control. ( G ) Summary graphs of relative abundance for Western blots shown above. ( H and I ) Western blot analysis of the cGAS/STING pathway molecules (cGAS, STING, p-TBK1, TBK1, p-IRF3, and IRF3) in the kidneys of Wistar and T2DN old male rats. n = 6 rats in each group. Data presented as mean ± SEM. Statistical analysis was performed using unpaired, 2-tailed Student’s t test. * P < 0.05, ** P < 0.01, **** P < 0.0001. NS, nonsignificant; a.u., arbitrary units.

Article Snippet: Male Wistar rats (12 and 50 weeks, nondiabetic control) were purchased from Charles River Laboratories.

Techniques: Immunohistochemical staining, Staining, Immunofluorescence, Microscopy, Western Blot, Control

Journal: Scientific Reports

Article Title: Retinal blood flow dysregulation precedes neural retinal dysfunction in type 2 diabetic mice

doi: 10.1038/s41598-021-97651-3

Figure Lengend Snippet: Average systemic and ocular parameters in db/db and db/m mice from 8 to 20 weeks of age. Significant increases were observed in body weight ( A ) and blood glucose ( B ) in db/db mice (n = 6) compared with nondiabetic db/m mice (n = 6) by two-way repeated measures ANOVA. In contrast, no significant differences were observed in systemic blood pressure ( C ), mean arterial blood pressure ( D ), intraocular pressure ( E ), and ocular perfusion pressure ( F ) between two animal groups during follow-up period. Data are expressed as the mean ± SEM; au = arbitrary unit; *P < 0.05 between groups; NS = not significant between groups.

Article Snippet: The 7-week-old male C57BL/KsJ-db/db mice (BKS.Cg-Dock7 m +/+Lepr db /J; n = 6) and db/m (congenic nondiabetic littermates, n = 6) control mice were acquired from Charles River Laboratories JAPAN, Inc. (Yokohama, Japan) one week before the study.

Techniques: